Aptamers containing thymidine 3'-O-phosphorodithioates: synthesis and binding to nuclear factor-kappaB

X Yang; S Fennewald; B A Luxon; J Aronson; N K Herzog; D G Gorenstein

doi:10.1016/s0960-894x(99)00600-9

Aptamers containing thymidine 3'-O-phosphorodithioates: synthesis and binding to nuclear factor-kappaB

Bioorg Med Chem Lett. 1999 Dec 6;9(23):3357-62. doi: 10.1016/s0960-894x(99)00600-9.

Authors

X Yang¹, S Fennewald, B A Luxon, J Aronson, N K Herzog, D G Gorenstein

Affiliation

¹ Sealy Center for Structural Biology, Department of Human Biological Chemistry & Genetics, The University of Texas Medical Branch at Galveston, 77555-1157, USA.

PMID: 10612599
DOI: 10.1016/s0960-894x(99)00600-9

Abstract

Aptamers targeting NF-kappaB containing thymidine 3'-O-phosphorodithioates in selected positions of an oligonucleotide duplex were synthesized. Binding affinities to NF-kappaB varied with the number and positions of the dithioate backbone substitutions. One of the aptamers showed specific binding to a single NF-kappaB dimer in cell culture extracts.

Publication types

Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, P.H.S.

MeSH terms

Base Sequence
Magnetic Resonance Spectroscopy
NF-kappa B / metabolism*
Oligonucleotides / chemical synthesis*
Oligonucleotides / chemistry
Oligonucleotides / metabolism
Organothiophosphorus Compounds / analysis*

Substances

NF-kappa B
Oligonucleotides
Organothiophosphorus Compounds

Abstract

Publication types

MeSH terms

Substances

Grants and funding